Human, Bacterial and Fungal Amplicon Collection and Processing for Sequencing

Human, Bacterial and Fungal Amplicon Collection and Processing for Sequencing

Blog Article

Sequencing taxonomic marker genes is a powerful tool to interrogate the composition of microbial communities.For example, bacterial and fungal community composition can be evaluated in parallel using the 16S ribosomal RNA gene for bacteria or the internal transcribed spacer region in fungi.These are conserved regions that are universal to a taxonomic clade, yet have undergone some Dryer Drum Bearing degree of evolution such that different lineages can be differentiated.Conserved regions are used for design of universal priming sites that allow amplification of the marker gene out of a mixed microbial community.

Here, we describe our standard operating procedure to collect and sequence 16S rRNA and ITS1 amplicons from human skin.We use the 16S rRNA V1-V3 region for skin Flip Flop Armless Sofa samples, as it has greater power for classifying common staphylococci in the skin.This protocol is adapted for 454 pyrosequencing of amplicons.